Analysis of In Situ Protease Activity in Chronic Adult Periodontitis Patients: Expression of Activated MMP‐2 and a 40 kDa Serine Protease
- 1 March 2000
- journal article
- Published by Wiley in The Journal of Periodontology
- Vol. 71 (3) , 353-360
- https://doi.org/10.1902/jop.2000.71.3.353
Abstract
Periodontitis is characterized by extensive destruction of the gingival tissues and associated supporting structures of the teeth. Although the pathogenesis of the various forms of this disease is not completely understood, host-derived proteases are believed to have an important role. In this study, we analyzed human tissue samples from chronic adult periodontitis patients to assess the levels of specific proteases and determine the effect of pH and tetracyclines on their activity. Gingival tissue samples were obtained from patients with chronic adult periodontitis (probing depths ranged from 5 to 9 mm) and periodontally healthy controls. Tissue extracts were prepared and analyzed for protease activity by zymography and Western blotting. Maximal protease activity from clinically normal and diseased tissues was observed at pH 8. Latent matrix metalloproteinase (MMP)-9 and MMP-2 were expressed in all samples examined, while active MMP-2 was detected only in tissues obtained from patients with clinical disease. The MMP activities were differentially inhibited by derivatives of tetracycline. At pH 6, a protease with a mass of approximately 40 kDa was observed in diseased samples. The enzymatic activity was inhibited by phenylmethylsulfonyl fluoride, suggesting it is a serine protease. The results of the current study substantiate the proposed role of host-derived proteases in the pathogenesis of chronic adult periodontitis. Specifically, they indicate that activated MMP-2 and a 40 kDa serine protease are involved in tissue destruction associated with this form of periodontal disease and also suggest that tissue pH influences protease activity in situ.Keywords
This publication has 25 references indexed in Scilit:
- Human Neutrophil Gelatinase and Associated Lipocalin in Adult and Localized Juvenile PeriodontitisJournal of Dental Research, 1996
- Collagenases and tissue inhibitors of metalloproteinases: a functional balance in tissue degradationOral Diseases, 1996
- Tetracyclines in the management of periodontal diseasesJournal of Clinical Periodontology, 1995
- Effects of Tetracyclines on Neutrophil, Gingival, and Salivary CollagenasesAnnals of the New York Academy of Sciences, 1994
- Possible functions of human neutrophil serine proteinases, medullasin and cathepsin G, in periodontal tissue breakdownJournal of Periodontal Research, 1993
- Microregional distributions of glucose, lactate, ATP and tissue pH in experimental tumours upon local hyperthermia and/or hyperglycaemiaZeitschrift für Krebsforschung und Klinische Onkologie, 1993
- Induction of metalloproteinase activity in human T-lymphocytesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1993
- Nature of periodontal diseases: Pathogenic mechanismsJournal of Periodontal Research, 1987
- The Bowman‐Birk inhibitor. Trypsin‐ and chymotrypsin‐inhibitor from soybeansInternational Journal of Peptide and Protein Research, 1985
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970