Nucleotide Sequence for a Novel Duck Alpha‐Globin Gene

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Abstract
Nucleotide sequence analysis of a recombinant c[complementary]DNA for a duck .alpha.-globin gene indicates a gene with novel features and evolutionary history. The duck .alpha.-globin double-stranded cDNA, treated with S1 nuclease and tailed with poly(dC), was inserted into the PstI restriction endonuclease site of pBR322 tailed with poly(dG). Nucleotide sequence analysis of the resulting recombinant cDNA indicates that it contains the translated region and all of the 3'' untranslated region for an .alpha.-globin gene. The sequence was determined by procedures designed especially for rapid analysis of pBR322 (PstI site) recombinant cDNA. This duck globin has sequences related to the chicken .alpha.-A globin in some regions but in other regions it is more closely related to another .alpha. globin found in anemic chickens.