The Structure of Sugar Chains of Japanese Quail Ovomucoid. The Occurrence of Oligosaccharides Not Expected from the Classical Biosynthetic Pathway for N-Glycans; A Method for the Assessment of the Structure of Glycans Present in Picomolar Amounts1

Publisher Website
Google Scholar
Abstract
While the structure of the major oligosaccharide of Japanese quail ovomucoid was reported earlier (Hase, S. et al . (1982) J. Biochem . 91 , 735–737), the structures of the minor oligosaccharide units were investigated for the first time in the present studies. For this purpose, the glycans of the protein were liberated from the polypeptide chain by hydrazinolysis. After N -acetylation, the reducing ends of the oligosaccharides obtained were coupled with 2-aminopyridine, and then the resulting fluorescent derivatives were purified by Bio-Gel P-2 column chromatography and reversed-phase HPLC. The chemical structures of two minor oligosaccharide units were determined with the aid of exoglycosidases, and by methylation analysis and Smith degradation. The results demonstrated that the ovomucoid contains the following two monoantennary glycans: Manα1-6(Gal β 1-4GlcNAc β 1-2Manα1-3)Man β 1-4GlcNAc β 1-4GlcNAc and Gal β 1-4GlcNAc β 1-2 Manα1-6(Manα1-3)Man β 1-4GlcNAc β 1-4GlcNAc The latter structure was not predicted by the classical metabolic pathway for the N -glycans to be formed. The structures of three additional minor heterosaccharides were deduced from their elution positions on HPLC together with the results of determination of their molecular sizes and the HPLC elution positions of their enzymatic degradation products. It is noteworthy that for the latter procedure for the estimation of the structures of oligosaccharides only minute quantities of glycans (several hundreds pmol) are required.

This publication has 0 references indexed in Scilit: