Localization of 5-hydroxytryptamine to neurons and endoneurial mast cells in rat sensory ganglia
- 1 February 1985
- journal article
- research article
- Published by Springer Nature in Journal of Neurocytology
- Vol. 14 (1) , 63-78
- https://doi.org/10.1007/bf01150263
Abstract
5-Hydroxytryptamine (5-HT) localization has been studied in the spinal and trigeminal ganglia of adult rats using immunofluorescence, peroxidase-antiperoxidase immunocytochemistry and [3H]5-HT uptake radioautography, the latter two at the ultrastructural level. Endoneurial mast cells, identified by alcian blue staining, formed 8 and 14% of all mast cells and neurons in spinal and trigeminal ganglia respectively and had a median diameter of 7.6 μm. Light and electron microscopic immunocytochemistry showed that these mast cells contained 5-HT-like immunoreactivity. Some 75% of them accumulated exogenous [3H]5-HTin vitro. A population of small-diameter neurons, which did not stain with alcian blue, was also labelled with anti-5-HT serum and accumulated [3H]5-HT. The possible roles of 5-HT in sensory ganglia are discussed.This publication has 45 references indexed in Scilit:
- MAST CELL CHANGES IN INH-INDUCED NEUROPATHY IN THE RATActa Pathologica Microbiologica Scandinavica, 2009
- Central serotonergic nerves project to the pial vessels of the brainNature, 1983
- Ultrastructural features of six types of neurons in rat dorsal root gangliaJournal of Neurocytology, 1983
- The occurrence of serotonergic nerves in the bovine corneaNeuroscience Letters, 1983
- Serotonin‐Containing Neurones in Vertebrate RetinasJournal of Neurochemistry, 1982
- Immunoreactive substance P and serotonin present in the same dense-core vesiclesNature, 1981
- High resolution analysis of [3H]2-deoxyglucose incorporation into neurons and glial cells in invertebrate ganglia: Histological processing of nervous tissue for selective marking of glycogenJournal of Neurocytology, 1981
- Reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibersJournal of Comparative Neurology, 1979
- Peptide neurons in the vagus, splanchnic and sciatic nerves*Acta Physiologica Scandinavica, 1978
- In vitro Studies on the Uptake of Biogenic Amines by Rat Mast CellsActa Physiologica Scandinavica, 1972