REGULATION OF CYTOCHROME P-450sccIN IMMATURE PORCINE GRANULOSA CELLS BY FSH AND ESTRADIOL
- 1 March 1983
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 112 (3) , 1156-1158
- https://doi.org/10.1210/endo-112-3-1156
Abstract
FSH, estradiol, or a combination of FSH and estradiol enhanced secretion of progesterone by primary cultures of immature porcine granulosa cells; they also increased the mitochondrial content of cytochrome P-450 and mitochondrial cholesterol side-chain cleavage activity. FSH or estradiol alone increased mitochondrial cytochrome P-450 levels and cholesterol side-chain cleavage activity to a similar extent. The combination of FSH and estradiol further increased cytochrome P-450 and side-chain cleavage activity. Our results demonstrate that FSH, estrogen and the combination of the hormones stimulate progestin synthesis by granulosa cells, at least in part, by increasing cholesterol side-chain cleavage enzyme levels. These observations suggest a dual hormonal regulation of the enzyme catalyzing the rate-limiting step in the biosynthetic pathway of steroid hormones.Keywords
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