Chloroplast‐released inhibitor of phenylalanine ammonia‐lyase from barley (Hordeum vulgare) seedlings

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Abstract
Isolated chloroplasts of barley seedlings (Hordeum vulgare L.) when kept in light, released a soluble, thermostable factor that inhibited phenylalanine ammonia‐lyase (PAL) in vitro. Highest inhibition was found when chloroplasts were suspended in PAL‐containing extract and kept in light. Efficiency of PAL activity inhibition did not depend greatly on the type of medium used for chloroplast isolation, nor on the composition of buffer in which the enzymatic activity was measured. It is proposed that in green tissues chloroplasts may participate in regulation of cytoplasmic PAL activity.