Direct enzyme transfer from lymphocytes is specific
Open Access
- 1 November 1983
- journal article
- letter
- Published by Springer Nature in Nature
- Vol. 306 (5938) , 75-77
- https://doi.org/10.1038/306075a0
Abstract
Lymphocytes are known to interact directly with other cells in vivo1,2 and in vitro3–5, and have recently been shown to transfer the lysosomal enzyme, β-glucuronidase, to fibroblasts from patients with an inherited deficiency of the enzyme6. This process requires cell–cell contact, is unaffected by inhibitors of ‘classical’ receptor-mediated endocytosis and is abolished by inhibitors of protein synthesis7. Although it is not yet known to what extent the transfer of enzymes by direct cellular interaction is a general phenomenon, a similar mechanism could possibly be involved in the transfer of other lysosomal enzymes in vivo8–10 and in the exchange of protein in vitro11. We show here that the direct transfer of enzymes from lymphocytes to fibroblasts is restricted to only certain lysosomal enzymes.Keywords
This publication has 30 references indexed in Scilit:
- Acquisition of β-glucuronidase activity by deficient fibroblasts during direct contact with lymphoid cellsJournal of Cell Science, 1982
- Direct transfer of a lysosomal enzyme from lymphoid cells to deficient fibroblastsNature, 1981
- Intercellular transfer of β-glucuronidase in chimeric miceJournal of Cell Science, 1979
- Crawling movements of lymphocytes on and beneath fibroblasts in cultureProceedings of the National Academy of Sciences, 1979
- Endothelial-lymphocyte interactions in vitro I. Adherence of nonallergised lymphocytesCellular Immunology, 1976
- Solitary cells and enzyme exchange in tetraparental miceNature, 1976
- Enzyme Replacement Therapy—An Experiment of Nature in a Chimeric Mannosidosis CalfPediatric Research, 1976
- Invitro correction of deficient human fibroblasts by β-glucuronidase from different human sourcesBiochemical and Biophysical Research Communications, 1974
- Sanfilippo Disease Type B: Enzyme Replacement and Metabolic Correction in Cultured FibroblastsScience, 1973
- The Defect in the Hurler and Scheie Syndromes: Deficiency of α-L-IduronidaseProceedings of the National Academy of Sciences, 1972