Intranuclear topological distribution of HIV‐1 trans‐activators
Open Access
- 22 June 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 305 (1) , 1-5
- https://doi.org/10.1016/0014-5793(92)80642-t
Abstract
Subcellular localization of human immunodeficiency virus type 1 (HIV‐1) Tat and Rev was examined using a confocal laser scanning microscope (CLSM). In transfected COS‐7 cells, Tat resided exclusively in the perinocleolar region, while Rev infiltrated fully into the nucleoli. The chimeric Tat in which the nucleolar targeting signal was replaced by that of Rev, which retains trans‐acting activity of Tat, remained still in the perinucleolar region as wild‐type Tat. Perinucleolar distribution of Tat protein suggests the existence of a novel nucleolar architecture that affects transcription.Keywords
This publication has 30 references indexed in Scilit:
- Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus.The Journal of cell biology, 1991
- Application of laser microtomography to in situ three-dimensional subcellular morphology.ACTA HISTOCHEMICA ET CYTOCHEMICA, 1991
- Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motifCell, 1989
- Functional similarity of HIV-I rev and HTLV-I rex proteins: Identification of a new nucleolar-targeting signal in rev proteinBiochemical and Biophysical Research Communications, 1989
- Major nucleolar proteins shuttle between nucleus and cytoplasmCell, 1989
- Sequence requirements for nucleolar localization of human T cell leukemia virus type I pX protein, which regulates viral RNA processingCell, 1988
- Microscopic tomography by laser scanning microscopy and its three‐dimensional reconstructionJournal of Microscopy, 1988
- Regulation of mRNA accumulation by a human immunodeficiency virus trans-activator proteinCell, 1987
- The trans-activator gene of the human T cell lymphotropic virus type III is required for replicationCell, 1986
- The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeatCell, 1985