Inhibition of Rat Brain Microsomal Na+,K+-ATPase by S-Adenosylmethionine
- 1 January 1984
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 42 (1) , 204-208
- https://doi.org/10.1111/j.1471-4159.1984.tb09718.x
Abstract
Rat brain microsomes were preincubated with S-adenosylmethionine (SAM), MgCl2, and CaCl2, then re-isolated, and the activity of Na+,K+-ATPase determined. SAM inhibited the Na+,K+-ATPase activity compared with microsomes subjected to similar treatment in the absence of SAM. A biphasic inhibitory effect was observed with a 50% decrease at a SAM concentration range of 0.4 μM-3.2 μM and a 70% reduction at a concentration range above 100 μM. Inclusion of either S-adenosylhomocysteine or 3-deazaadenosine in the preincubations prevented the SAM inhibition of Na+,K+-ATPase activity. The inhibition by SAM appeared to be Mg2+- or Ca2+-dependent.Keywords
This publication has 19 references indexed in Scilit:
- Phospholipid metabolism, calcium flux, and the receptor-mediated induction of chemotaxis in rabbit neutrophils.The Journal of cell biology, 1982
- Regulation of rat brain (Na+ + K+)-ATPase activity by cyclic AMPBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- Modulation of ATPase activities of human erythrocyte membranes by free fatty acids or phospholipase A2The Journal of Membrane Biology, 1982
- The (non) specificity in the lipid-requirement of calcium- and (sodium plus potassium)-transporting adenosine triphosphatasesLife Sciences, 1981
- Phospholipid Methylation and Biological Signal TransmissionScience, 1980
- Identification and Properties of Methyltransferases That Synthesize Phosphatidylcholine in Rat Brain SynaptosomesJournal of Neurochemistry, 1980
- Phospholipid methylation affects immunoglobulin E-mediated histamine and arachidonic acid release in rat leukemic basophilsBiochemical and Biophysical Research Communications, 1980
- Increased Ca2+-ATPase activity associated with methylation of phospholipids in human erythrocytesBiochemical and Biophysical Research Communications, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Solubilization and properties of a membrane-bound enzyme from rat brain catalyzing a base-exchange reactionBiochemical and Biophysical Research Communications, 1973