A simple and efficient method for site-directed mutagenesis with double-stranded plasmid DNA

Open Access

25 August 1993

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 21 (17) , 3977-3980
https://doi.org/10.1093/nar/21.17.3977

Abstract

A general, simple and efficient method for preparing site-specific mutations in double-stranded plasmid DNA without the need for special plasmlds, bacterial strains or reagents Is described. Only one synthetic oligonucleotlde for each mutation is required, subclonlng Is unnecessary and a high efficiency of mutation (58–97%) was obtained. If two synthetic oligonucleotide primers are used, two separate mutations can be simultaneously created In a single reaction tube.

Keywords

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