Somatic Embryogenesis in Carrot Cell Suspension

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Abstract
Acrylamide gel electrophoresis and affinity chromatography on oligo(dT)-cellulose of single- and double-labelled RNA were used to detect changes in the synthesis of rRNA and poly(A)$RNA during undifferentiated growth of carrot cell suspension in a medium containing the auxin, 2,4- dichlorophenoxyacetic acid and during embryogenesis in an auxin-free medium. The rate of rRNA synthesis in the embryogenic cells was generally lower than that in the non-embryogenic cells. On the other hand, embryogenic cells synthesized poly(A)$RNA at a higher rate than the nonembryogenic cells during an experimental period of 96 h. These results identify a role for mRNA synthesis in somatic embryogenesis in carrot cell suspension which may be useful for further studies on the regulation of this developmental programme.

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