Gene expression of type I phospholipase A2 in pancreatic beta cells
- 16 December 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 295 (1-3) , 110-112
- https://doi.org/10.1016/0014-5793(91)81397-q
Abstract
Messenger RNA from intact rat pancreatic islets, or from transformed hamster beta (HIT) cells, hybridized with the cDNA probe for type I (but not type II) phospholipase A2. The levels of phospholipase A2 mRNA increased in islets from fasted rats: they decreased in islets cultured in a high glucose concentration (control values at 5.5 mM glucose = 150±6% of those at 22 mM) which impaired subsequent insulin secretion (reduction in second‐phase release = 70±11%). These studies uniquely demonstrate that type I phospholipase A2 is expressed specifically in beta cells and that nutrient availability modulates transcript levels, an effect which could contribute to the detrimental influence of prolonged hyperglycemia on islet function.Keywords
This publication has 24 references indexed in Scilit:
- Abnormal Sensitivity to Glucose of Human Islets Cultured in a High Glucose Medium: Partial Reversibility after an Additional Culture in a Normal Glucose MediumJournal of Clinical Endocrinology & Metabolism, 1991
- Starvation decreases insulin secretion, prostaglandin E2 production and phospholipase A2 activity in rat pancreatic isletsJournal of Endocrinology, 1990
- cDNA cloning and sequence determination of rat membrane-associated phospholipase A2Biochemical and Biophysical Research Communications, 1989
- Presence of pancreatic-type phospholipase A2 mRNA in rat gastric mucosa and lungBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1989
- Membrane phospholipid turnover as an intermediary step in insulin secretionThe American Journal of Medicine, 1988
- Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloningBiochemistry, 1987
- [20] Isolation of RNA using guanidinium saltsPublished by Elsevier ,1987
- Ether-Linked Lysophospholipids Initiate Insulin Secretion: Lysophospholipids May Mediate Effects of Phospholipase A2 Activation on Hormone ReleaseDiabetes, 1986
- Role of insulin secretagogues in the regulation of somatostatin binding by isolated rat islets.Journal of Clinical Investigation, 1980
- Effect of starvation on insulin and glucagon release from isolated islets of Langerhans of the ratMetabolism, 1969