Synthesis of p-Isothiocyanatophenyl 3-O-(3,6-Dideoxy-alpha-D-xylo-hexopyranosyl)-alpha-D-mannopyranoside.

Abstract
A synthesis of the disaccharide derivative p-isothiocyanatophenyl 3-O-(3,6-dideoxy-.alpha.-D-xylo-hexopyranosyl)-.alpha.-D-mannopyranoside, corresponding to the Salmonella O-factor 4, is described. The p-isothiocyanatophenyl group is useful for attaching the abequosylmannosyl disaccharide unit to free amino groups in a protein. The glycosylation step in the synthetic sequence was performed using the Helferich procedure and 2,4-di-0-p-nitrobenzoyl-.alpha.-abequosyl bromide as the glycosylating reagent.

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