Molecular weight of bacteriorhodopsin solubilized in Triton X-100.
- 1 July 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (7) , 2803-2804
- https://doi.org/10.1073/pnas.74.7.2803
Abstract
Bacteriorhodopsin from Halobacterium halobium was solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin is monomeric under the conditions of solubilization with a MW of 24,250 .+-. 2000 and binds about 1 micelle of Triton X-100.Keywords
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