The Use of Picric Acid with the Gram Stain in Plant Cytology

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Abstract
The technic described involves the use of a saturated solution of picric acid in absolute alcohol in the process of dehydration following the gentian-violet-iodine stain as applied to plant material. The method is suitable for both paraffin sections and smears of pollen mother cells fixed in Navashin''s or Flemming''s solutions. Differentiation in clove oil is very easy since cytoplasm destains immediately, while chromatic material destains very slowly following picric acid. Chromosomes are stained more distinctly than with the usual Gram stain and do not fade.

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