Abstract
The ATP‐dependent breakdown of mitochondria‐containing stroma proceeds via the ubiquitin‐requiring pathway. The proteolysis is linked to a large ATP‐cleaved consumption amounting to 1 ATP per peptide bond or more. Proteins of mitochondria‐containing stroma are much better substrates of ATP‐ubiquitindependent proteolysis than heat‐denatured ones. Hemin suppresses both proteolysis and ATP hydrolysis.