Methods for detection of reactive metabolites of oxygen and nitrogen: in vitro and in vivo considerations
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- 1 March 2004
- journal article
- review article
- Published by American Physiological Society in American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Vol. 286 (3) , R431-R444
- https://doi.org/10.1152/ajpregu.00361.2003
Abstract
Facile detection of reactive oxygen and nitrogen species in biologic systems is often problematic. This is a result of the numerous cellular mechanisms, both enzymatic and nonenzymatic involved in their catabolism/decomposition, the complex and overlapping nature of their reactivities, as well as the often limited intracellular access of detector systems. This review describes approaches to the direct and indirect measurement of different reactive metabolites of oxygen and nitrogen. Particular attention to a method's applicability for in vivo determinations will be addressed.Keywords
This publication has 145 references indexed in Scilit:
- Cytochrome c Is a Potent Catalyst of Dichlorofluorescin Oxidation: Implications for the Role of Reactive Oxygen Species in ApoptosisBiochemical and Biophysical Research Communications, 2001
- S-Nitrosothiol Formation in Blood of Lipopolysaccharide-Treated RatsBiochemical and Biophysical Research Communications, 2000
- Validation of Lucigenin as a Chemiluminescent Probe to Monitor Vascular Superoxide as Well as Basal Vascular Nitric Oxide ProductionBiochemical and Biophysical Research Communications, 1999
- Quantification of Superoxide Radical Formation in Intact Vascular Tissue Using aCypridinaLuciferin Analog as an Alternative to LucigeninBiochemical and Biophysical Research Communications, 1998
- Acceleration of oxidative stress-induced endothelial cell death by nitric oxide synthase dysfunction accompanied with decrease in tetrahydrobiopterin contentLife Sciences, 1997
- Spin Trapping of Superoxide Radicals and Peroxynitrite by 1-Hydroxy-3-carboxy-pyrrolidine and 1-Hydroxy-2,2,6,6-tetramethyl-4-oxo-piperidine and the Stability of Corresponding Nitroxyl Radicals Towards Biological ReductantsBiochemical and Biophysical Research Communications, 1997
- Peroxidative oxidation of leuco-dichlorofluorescein by prostaglandin H synthase in prostaglandin biosynthesis from polyunsaturated fatty acidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996
- Cautionary note for DMPO spin trapping in the presence of iron ionBiochemical and Biophysical Research Communications, 1990
- The use of acetylated ferricytochrome C for the detection of superoxide radicals produced in biological membranesBiochemical and Biophysical Research Communications, 1975
- Direct demonstration of superoxide anion production during the oxidation of reduced flavin and of its catalytic decomposition by erythrocupreinBiochemical and Biophysical Research Communications, 1969