ADP‐ribosylation of the 78‐kDa glucose‐regulated protein during nutritional stress
- 1 December 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 186 (1-2) , 205-211
- https://doi.org/10.1111/j.1432-1033.1989.tb15196.x
Abstract
Starvation of a mouse hepatoma cell line, Hepa, for any essential amino acid results in the mono-ADP-ribosylation of an 80-kDa protein, P80. The ADP-ribose acceptor and its putative precursor were identified in two-dimensional gel patterns and isolated by electroelution. Amino-terminal sequence analysis showed they were the 78-kDa glucose-regulated protein, GRP78. Starvation of Hepa cells for tryptophan or glucose stimulated the relative rate of synthesis, and the ADP-ribosylation of GRP78. Inhibition of N-linked glycosylation by treatment with tunicamycin, 2-deoxy-D-glucose or glucosamine stimulated the synthesis of non-ADP-ribosylated GRP78 up to sixfold with relatively little effect on its ADP-ribosylation. Both forms were identified in mouse liver, lung, heart, kidney, spleen and brain.This publication has 35 references indexed in Scilit:
- Adp‐Ribosylation of Guanyl Nucleotide‐Binding Regulatory Proteins by Bacterial ToxinsPublished by Wiley ,1988
- Formation of a functional ribosome-membrane junction during translocation requires the participation of a GTP-binding protein.The Journal of cell biology, 1986
- Translational control of ADP‐ribosylation in eucaryotic cellsEuropean Journal of Biochemistry, 1986
- On the nature of cellular ADP-ribosyltransferase from rat liver specific for elongation factor 2Biochemical and Biophysical Research Communications, 1986
- Speculations on the functions of the major heat shock and glucose-regulated proteinsCell, 1986
- Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas.The Journal of cell biology, 1986
- Uncoupling Translocation from Translation: Implications for Transport of Proteins Across MembranesScience, 1986
- Uncoating ATPase is a member of the 70 kilodalton family of stress proteinsCell, 1986
- ADP‐ribosyltransferase from beef liver which ADP‐ribosylates elongation factor‐2FEBS Letters, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970