Artefactual Origins of Cyclic AMP in Higher Plant Tissues
Open Access
- 1 October 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 91 (2) , 624-628
- https://doi.org/10.1104/pp.91.2.624
Abstract
A highly sensitive radioimmunoassay has been used to determine the levels of adenosine 3′,5′-cyclic monophosphate (cAMP) in five higher plants (Lactuca sativa, Helianthus annuus, Oryza sativa, Pinus pinaster, Nicotiana tabacum). Particular attention was paid to the three main sources of errors in the characterization of cAMP in plants: presence of interfering substances in plant tissues; possible artefactual formation of cAMP from endogenous ATP during extraction, purification, and assay; and microbial origin of cAMP. In all the tested tissues, the cAMP level was below the detection limit of 0.5 picomole per gram fresh weight, a value much lower than those reported for similar materials of the same species in many previous studies. This result is not in favor of cAMP-dependent regulations in higher plants.This publication has 12 references indexed in Scilit:
- Radioimmunoassay of cyclic AMP can provide a highly sensitive assay for adenylate cyclase, even at very high ATP concentrationsAnalytical Biochemistry, 1985
- The quantitation of adenosine 3′:5′‐cyclic monophosphate in cultured tobacco tissue by mass spectrometryFEBS Letters, 1981
- Stabilization of adenine nucleotide ratios at various values by an oxygen limitation of respiration in germinating lettuce (Lactuca sativa) seedsBiochemical Journal, 1980
- Isolation, characterization and distribution of adenosine 3′:5′-cyclic monophosphate from Pinus radiataBiochemical Journal, 1978
- Attempts to Detect Cyclic Adenosine 3′:5′-Monophosphate in Higher Plants by Three Assay MethodsPlant Physiology, 1976
- L'AMP cyclique dans les graines et les plantules d'orge et la contamination bactérienne ou fongiqueBiochimie, 1975
- Adenosine 3′, 5′-cyclic monophosphate in higher plants: Assay, distribution and age-dependencyBiochimica et Biophysica Acta (BBA) - General Subjects, 1974
- Adenosine 3′,5′ cyclic monophosphate assay at 10−15 mole levelAnalytical Biochemistry, 1973
- Evidence for the presence of 3′, 5′-cyclic AMP in plant tissuesBiochemical and Biophysical Research Communications, 1973
- Separation of cyclic 3′,5′-nucleoside monophosphates from other nucleotides on aluminum oxide columns application to the assay of adenyl cyclase and guanyl cyclaseAnalytical Biochemistry, 1971