CALCIUM AUGMENTATION OF ENZYME LEAKAGE FROM MOUSE SKELETAL-MUSCLE AND ITS POSSIBLE SITE OF ACTION
- 1 January 1978
- journal article
- research article
- Vol. 20 (2) , 317-329
Abstract
Creatine kinase efflux from isolated mouse skeletal muscle was monitored in the presence of various concentrations of Ca2+ or La3+ for 5 h. Ca2+ levels (10-3 to 10-2 M) augmented enzyme leakage to greater than 300% above control. La3+, which may displace Ca2+ from sarcolemmal and t[transverse]-tubule membranes, depressed enzyme efflux at concentrations between 10-5 to 10-3 M. Extracellular Ca2+ mediates in vitro enzyme efflux and may act on the sarcolemma and its extensions. The regulation of enzyme leakage in various muscle disorders was discussed.This publication has 3 references indexed in Scilit:
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- Differential effects of calcium ion concentration on cell fusion, cell division and creatine kinase activity in muscle cell culturesExperimental Cell Research, 1976
- AN IMPROVED PROCEDURE FOR SERUM CREATINE PHOSPHOKINASE DETERMINATION1967