Whole-blood Hemoglobin Determinations: A Comparison of Methodologies

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Abstract
The Coulter Model S provides an accurate measurement of total hemoglobin (Hb) content in peripheral blood even when elevated levels of carboxyhemoglobin (HbCO) are present.Commerical standards and diluents for spectrophotometric determinations of cyanmethemoglobin (HiCN) are variable inquality. All diluents studied produce turbidity in some samples, and this may explain why manual Hb determinations tend to be higher than hemoglobin measurement on the Coulter S. The most reliable diluent was the reagent described by VanKampen and Zijlstra. Its reliability is enhanced by centrifugationof sample in diluent. The use of a narrow-band-passspectrophotometer for routine HiCN determinations in aclinical laboratory is not recommended because of its sensitivity to turbidity and HbCO.

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