Requirement of G M2 ganglioside activator for phospholipase D activation
- 13 October 1998
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 95 (21) , 12249-12253
- https://doi.org/10.1073/pnas.95.21.12249
Abstract
Sequence analysis of a heat-stable protein necessary for the activation of ADP ribosylation factor-dependent phospholipase D (PLD) reveals that this protein has a structure highly homologous to the previously known GM2 ganglioside activator whose deficiency results in the AB-variant of GM2 gangliosidosis. The heat-stable activator protein indeed has the capacity to enhance enzymatic conversion of GM2 to GM3 ganglioside that is catalyzed by β-hexosaminidase A. Inversely, GM2 ganglioside activator purified separately from tissues as described earlier [Conzelmann, E. & Sandhoff, K. (1987) Methods Enzymol. 138, 792–815] stimulates ADP ribosylation factor-dependent PLD in a dose-dependent manner. At higher concentrations of ammonium sulfate, the PLD activator protein apparently substitutes for protein kinase C and phosphatidylinositol 4,5-bisphosphate, both of which are known as effective stimulators of the PLD reaction. The mechanism of action of the heat-stable PLD activator protein remains unknown.Keywords
This publication has 32 references indexed in Scilit:
- Reconstitution of GTP-γ-S-dependent phospholipase D activity with ARF, RhoA, and a soluble 36-kDa proteinFEBS Letters, 1996
- Potential Role of Protein Phosphorylation in GTP-γ-S-Dependent Activation of Phospholipase DBiochemical and Biophysical Research Communications, 1995
- Low Molecular Weight GTP-binding Proteins in HL-60 GranulocytesPublished by Elsevier ,1995
- The Mouse Gene Encoding the GM2 Activator Protein (Gm2a): cDNA Sequence, Expression, and Chromosome MappingGenomics, 1994
- Two distinct members of the ADP-ribosylation factor family of GTP-binding proteins regulate cell-free intra-golgi transportCell, 1992
- Activator proteins and topology of lysosomal sphingolipid catabolismBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1992
- Characterization of full‐length cDNAs and the gene coding for the human GM2 activator proteinFEBS Letters, 1991
- Isolation and expression of a full-length cDNA encoding the human GM2 activator proteinBiochemical and Biophysical Research Communications, 1991
- Rapid purification of protein kinase C by high performance liquid chromatographyBiochemical and Biophysical Research Communications, 1986
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976