ALTERATION IN LYSOSOME SUPRAVITAL STAINING AS A MARKER OF HYDROXYUREA-INDUCED CYTO-TOXICITY AND ITS MODIFICATION BY RADICAL SCAVENGERS IN L5178Y-CELLS IN CULTURE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 30  (5) , 541-545
Abstract
Supravital staining of lysosomal membranes with euchrysine was employed to study hydroxyurea (HU)-induced toxic side effects in L5178Y [mouse] lymphoblasts in culture. Exposure of cells to 0.1-1.0-10.0 mM HU-induced progressive increase in the proportion of cells without detectable lysosomal fluorescence. This effect preceded the occurrence of non-viable cells, determined by trypan-blue exclusion test. Addition of .alpha.-tocopherol, acetylosalicylic acid, sodium benzoate or ascorbic acid to the culture medium afforded a concentration-dependent modification of lysosomal response to HU treatment. The fluorescence technique of supravital lysosome staining can be a useful test in studies on the toxic side effects of free radical-forming drugs and their amelioration by radical scavengers.