Preparation and properties of bacterial chromatophores entrapped in polyacrylamide
- 1 October 1976
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 18 (10) , 1425-1432
- https://doi.org/10.1002/bit.260181009
Abstract
The immobilization of Rhodospirillum rubrum chromatophores was successfully performed by entrapping them in polyacrylamide. Their photophosphorylating activity was about 40% of native chromatophores. The temperature and pH optima for immobilized chromatophores were similar to the native photosynthetic apparatus and kinetic parameters showed that the rate of photophosphorylation in polyacrylamide particles was diffusion controlled. Light penetration of the gel particles was not a limiting parameter. Immobilization considerably increased the stability of the chromatophores towards denaturation.This publication has 7 references indexed in Scilit:
- Photosynthetic regeneration of ATP using bacterial chromatophoresBiotechnology & Bioengineering, 1976
- Respiratory properties of rat liver mitochondria immobilized on an alkylsilylated glass surface.Journal of Biological Chemistry, 1975
- Continuous production of urocanic acid by immobilized Achromobacter liquidum cellsBiotechnology & Bioengineering, 1974
- Protein-containing polyacrylamide gels: Their use as immunoadsorbents of high capacityImmunochemistry, 1971
- A DNA-Acrylamide Gel Column for Analyzing Proteins That Bind to DNA, I. DNA PolymeraseProceedings of the National Academy of Sciences, 1970
- Antibody in polyacrylamide gel, a solid phase reagent for radioimmunoassayImmunochemistry, 1969
- Studies on bacterial photophosphorylation V. Stoichiometry and kinetics of photophosphorylation and adenosine 5′-triphosphatase and effects of divalent metal ionsBiochimica et Biophysica Acta, 1962