Application of a single-cell isolation technique to studies of carbon assimilation by the subtropical silicoflagellate Dictyocha perlae vis

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Abstract
A single-cell isolation technique was used to study the assimilation of 14CO2 by natural populations of the silicoflagellate D. perlaevis. The method uses a concentrated natural sample and a high activity of radioisotope. The technique facilitates physiological studies of certain species which cannot be conveniently studied in the laboratory due to difficulty in maintaining laboratory cultures. Data obtained from a subtropical embayment are used to illustrate the application of the method, and include in particular a study of C partitioning patterns into major polymers over the course of a diel cycle.