Multi-Virulence-Locus Sequence Typing Clarifies Epidemiology of Recent Listeriosis Outbreaks in the United States
Open Access
- 1 October 2005
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 43 (10) , 5366-5368
- https://doi.org/10.1128/jcm.43.10.5291-5294.2005
Abstract
We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a LightCycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.Keywords
This publication has 20 references indexed in Scilit:
- Microbiological Aspects of the Investigation That Traced the 1998 Outbreak of Listeriosis in the United States to Contaminated Hot Dogs and Establishment of Molecular Subtyping-Based Surveillance for Listeria monocytogenes in the PulseNet NetworkJournal of Clinical Microbiology, 2005
- Genetic Markers Unique to Listeria monocytogenes Serotype 4b Differentiate Epidemic Clone II (Hot Dog Outbreak Strains) from Other LineagesApplied and Environmental Microbiology, 2004
- Multilocus Sequence Typing of Listeria monocytogenes by Use of Hypervariable Genes Reveals Clonal and Recombination Histories of Three LineagesApplied and Environmental Microbiology, 2004
- Multi-Virulence-Locus Sequence Typing ofListeria monocytogenesApplied and Environmental Microbiology, 2004
- MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignmentBriefings in Bioinformatics, 2004
- Molecular typing of pathogens.2003
- Regulation of virulence genes in ListeriaInternational Journal of Medical Microbiology, 2001
- PulseNet: The Molecular Subtyping Network for Foodborne Bacterial Disease Surveillance, United StatesEmerging Infectious Diseases, 2001
- PulseNet: The Molecular Subtyping Network for Foodborne Bacterial Disease Surveillance, United StatesEmerging Infectious Diseases, 2001
- Summary of a Workshop on the Clone Concept in the Epidemiology, Taxonomy, and Evolution of the Enterobacteriaceae and other BacteriaThe Journal of Infectious Diseases, 1983