Purification of a 32.5 kDa monomeric sulfotransferase from rat liver with activity for bile acids and phenolic steroids
- 27 October 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 207 (2) , 193-197
- https://doi.org/10.1016/0014-5793(86)81487-9
Abstract
Both bile acid and phenolic steroid sulfotransferase activities in rat liver cytosol have previously been identified in fractions corresponding to apparent molecular masses of 60–70 and 30–35 kDa. We purified the latter activity corresponding to a monomeric protein. Activity for bile acids and phenolic steroids co-eluted on sequential chromatography on Sephadex G-75 sf, Affigel blue, chromatofocusing and hydroxyapatite. The protein was homogeneous on SDS-PAGE (32.5 kDa)Keywords
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