Cloning and sequence of cDNA coding for alpha 1-antitrypsin.
- 1 November 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (11) , 6826-6830
- https://doi.org/10.1073/pnas.78.11.6826
Abstract
Recombinant plasmids containing human and baboon c[complementary]DNA were screened for .alpha.1-antitrypsin, a major serine protease inhibitor present in blood. One plasmid, designated pBa.alpha.1a2, contained a cDNA insert of 1352 base pairs coding for the baboon inhibitor. It included 45 nucleotides that code for 15 amino acids present in the amino-terminal signal sequence of the protein, 1182 nucleotides that code for 394 amino acids in the mature protein, a stop codon and a noncoding region of 76 nucleotides. Comparison of the amino acid sequences of baboon .alpha.1-antitrypsin, human antithrombin III and chicken ovalbumin indicated that these 3 proteins are about 30% homologous. A 2nd plasmid, designated pH.alpha.1a1, contained a human cDNA insert of 306 base pairs. This plasmid coded for 69 amino acids present in the carboxyl-terminal region of human .alpha.1-antitrypsin. The human and baboon cDNA and their amino acid sequences are > 96% homologous.This publication has 33 references indexed in Scilit:
- Studies on the oligosaccharide chains of human alpha 1-protease inhibitor. II. Structure of oligosaccharides.Journal of Biological Chemistry, 1980
- Interaction of human α-1-antitrypsin with porcine trypsinBiochemistry, 1978
- Sequence data handling by computerNucleic Acids Research, 1977
- The abnormality of the S variant of human α-1-antitrypsinBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976
- A simple method for DNA restriction site mappingNucleic Acids Research, 1976
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976
- Amino acid substitution Glu→Lys in α1‐antitrypsin PiZFEBS Letters, 1976
- Molecular abnormality of human alpha1-antitrypsin variant (Pi-ZZ) associated with plasma activity deficiency.Proceedings of the National Academy of Sciences, 1976
- Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresisBiochemistry, 1975
- Purification and properties of normal human α1-antitrypsinArchives of Biochemistry and Biophysics, 1973