Detection of pathogenesis-related proteins (PR or b) and of other proteins in the intercellular fluid of hypersensitive plants infected with tobacco mosaic virus

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Abstract
In tobacco mosaic virus infected hypersensitive Nicotiana species, the pathogenesis-related (PR) or b proteins were found with other proteins in the intercellular fluid of leaf tissue. Analysis of leaves from mock-inoculated plants did not reveal the presence of detectable amounts of proteins in the intercellular fluid. The presence of proteins in the intercellular fluid seems widespread since 10 proteins were detected in tobacco mosaic virus infected Chenopodium quinoa Willd. These proteins were found not only in inoculated tissue but also in the intercellular fluid of uninoculated upper leaves. A 2-dimensional gel electrophoretic analysis of intercellular fluid proteins from N. tabacum L. cv. Xanthi-nc infected leaves showed that the relative MW of protein b2 is larger (14,700) than the one of b1 and b3 (14,200). Other proteins, with MW 12,500-36,300, could also be detected. The presence of rather larger amounts of proteins, including the well-characterized b proteins, in the intercellular fluid of infected hypersensitive leaves probably reflects overall changes in cell-to-cell interactions and in the cell wall metabolism.