Abstract
Talaromyces flavus produced a metabolite that retarded radial growth and killed microsclerotia of Verticillium dahliae in vitro. Microsclerotia of two isolates of V. dahliae were killed (LD95) in less than 3 hr in vitro. Maximum titer of the metabolite was produced by T. flavus after 5 days in a high-carbon liquid medium that was shaken at 120 rpm and incubated at 27 C. The metabolite was also able to kill microsclerotia in each of the three sterile soils and in sand, indicating that the physical components of the soils did not inactivate the metabolite. The production of this metabolite may be involved in the biological control of V. dahliae by T. flavus.

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