Transforming and nontransforming growth factors are present in medium conditioned by fetal rat calvariae.
- 1 November 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (21) , 7335-7339
- https://doi.org/10.1073/pnas.82.21.7335
Abstract
Conditioned medium recovered from fetal rat calvarial cultures contains an autocrine factor termed bone-derived growth factor (BDGF); this factor has been purified by acid extraction, gel-permeation chromatography, and two reversed-phase HPLC steps and examined for mitogenicity on normal rat kidney fibroblasts (NRK, clone 49F). HPLC-purified BDGF caused a dose-related increase in cell number, DNA content, and [3H]thymidine incorporation into acid-insoluble material. Since highly purified HDGF appeared less mitogenic than cruder preparations, the latter were tested for additional growth factors, with particular attention to those required for anchorage-independent colony formation in soft agar. BDGF did not displace 125I-labeled epidermal growth factor (EGF) in a radioligand-receptor assay, indicating the absence of EGF and transforming growth factor .alpha. (TGF-.alpha.). Without EGF, no BDGF preparation induced NRK cells to form soft agar colonies. However, calvarial conditioned medium contained a factor which, like TGF-.beta., induced large soft-agar colonies in the presence of EGF; this TGF-.beta.-like factor did not copurify with BDGF. Polyclonal antibodies against platelet-derived growth factor did not neutralize the effects of BDGF on NRK cells. BDGF is a potent mitogen for nonskeletal-tissue-derived fibroblasts. Although crude BDGF preparations do contain TGF-.beta., BDGF is distinct from this factor and others necessary for NRK cell transformation to anchorage-independent growth.This publication has 36 references indexed in Scilit:
- Transforming growth factor-β controls receptor levels for epidermal growth factor in NRK fibroblastsCell, 1984
- Effect of bone-derived growth factor on DNA, RNA, and proteoglycan synthesis in cultures of rabbit costal chondrocytesMetabolism, 1982
- Human skeletal growth factor: characterization of the mitogenic effect on bone cells in vitroBiochemistry, 1982
- Extracts of bone contain a potent regulator of bone formationBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- Chemical and biological properties of a growth factor from human‐cultured osteosarcoma cells: Resemblance with platelet‐derived growth factorJournal of Cellular Physiology, 1980
- Effect of fibroblast growth factor on cultured fetal rat calvariaMetabolism, 1980
- Epidermal Growth Factor1979
- The platelet-derived growth factorCell, 1978
- Epithelioid and fibroblastic rat kidney cell clones: Epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformationJournal of Cellular Physiology, 1978
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976