Vinylpyrrolidone-Vinyl Acetate Copolymers as Mounting Media for Azo and Other Dyes
- 1 January 1962
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 37 (2) , 109-110
- https://doi.org/10.3109/10520296209114582
Abstract
Vinylpyrrolidone-vinyl acetate copolymers (Antara Chemicals, General Aniline and Film Corp., New York N. Y., PVP/VA, E-735 and E-635) were employed as mounting media by adition of ethanol-water to the concentrated ethanolic solution of these plastics (25% plastic in 50% ethanol). The E-735 copolymer was frequently employed and is specifically recommended because it exhibits the highest degree of water tolerance. This type of mounting medium was found to be especially satisfactory in the preservation of azo, oxidation, and other histochemically derived dyes. The medium is useful also in the preservation of stains for fat and certain metachromatic dyes. The inexpensive nature of this mountant and its ease of application recommend it as a useful substitute for glycerol-gelatin.Keywords
This publication has 1 reference indexed in Scilit:
- THE RELATIONSHIP BETWEEN FIXATION AND TECHNIQUES FOR THE HISTOCHEMICAL LOCALIZATION OF HYDROLYTIC ENZYMESJournal of Histochemistry & Cytochemistry, 1958