Influence of Steroids Upon Osmotic Pressure Changes in Isolated Frog Skin Pouches.

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Abstract
The influence of dl-aldosterone, 2-methyl-9[alpha]-fluorohydrocortone, prednisone and prednisolone upon osmotic pressures, as determined with the Fiske osmometer, inside of isolated frog skin pouches in Ringer bath was tested. A considerable increase in the number of milliosmols per liter and an increase in total fluid volume as a result of the action of the first two steroids were observed. The other steroids had no significant effect. The Na and K concentrations remained unchanged in the treated as compared to the untreated skin pouches. The increase in the osmotic pressure is most likely due to the release of organic molecules from the frog skin, which are under investigation. Steroid promoted water and electrolyte transfer may be related to such mechanisms.