Assignment of resonances in the phosphorus-31 NMR spectrum of d(GGAATTCC) by regiospecific labeling with oxygen-17
- 1 November 1984
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 23 (23) , 5523-5527
- https://doi.org/10.1021/bi00318a022
Abstract
The chemical synthesis of the octanucleotide d-(GGAATTCC) in which each of the phosphate groups is sequentially replaced by an 17O-containing phosphate group using a polymer-supported phosphoramidite method is described. All 7 phosphorus resonances in the 31P spectrum of d(GGAATTCC) can be resolved. Assignment of these resonances to a particular phosphate group in the chain is possible because labeling of a phosphate with 17O causes its particular signal to disappear from the spectrum. Phosphate residues toward the middle of the octamer have 31P NMR shifts similar to those found in polydeoxynucleotides, whereas those toward the ends resemble those of dinucleoside phosphates. These data are interpreted in terms of less flexibility of the phosphate groups in the center of the molecule as compared to those at the ends.This publication has 5 references indexed in Scilit:
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