A GENERAL METHOD FOR THE MONOXENIC CULTIVATION OF THE DAPHNIDAE
Open Access
- 1 October 1970
- journal article
- Published by University of Chicago Press in The Biological Bulletin
- Vol. 139 (2) , 321-332
- https://doi.org/10.2307/1540087
Abstract
Fourteen species of the family Daphnidae have been established under continuous monoxenic cultivation utilizing Chlamydomonas reinhardii as sole food organism in a medium consisting of calcium acetate, antibiotics, albumin, trace elements and the water soluble vitamins, folic acid, B12, calcium pantothenate, choline, pyridoxal, inositol, thiamin, nicotinamide, riboflavin, biotin and putrescine. The Daphnidae under cultivation include Daphnia magna, D. pulex, D. galeata mendotae, D. laevis, D. dubia, D. retrocurva, D. parvula, D. ambigua, D. catawba, Moina macrocopa Scapholeberis mucronata, Simocephalus serrulatus, Ceriodaphnia reticulata, and C. quadrangula. The requirements for vitamins for some species are more complex than for others. The complete medium is superior for all but Scapholeberis mucronata and markedly increases the lifespan and fertility of Moina macrocopa.Keywords
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