2-Aminopurine Inhibits the Double-Stranded RNA-Dependent Protein Kinase BothIn VitroandIn Vivo
- 1 October 1993
- journal article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 13 (5) , 323-328
- https://doi.org/10.1089/jir.1993.13.323
Abstract
The autophosphorylation of interferon (IFN)-induced double-stranded RNA-dependent p68 protein kinase (PKR) and phosphorylation of the alpha-subunit of the translation initiation factor eIF-2 were inhibited by 10 mM 2-aminopurine in vitro. High concentrations of ATP overcame the inhibition. Kinetic studies indicated that 2-aminopurine is a competitive inhibitor with respect to ATP, suggesting that these two molecules bind the same site on the kinase. Treatment of HeLa cells with poly(I):poly(C) stimulated PKR autophosphorylation in vivo. The stimulated activity was inhibited by 10 mM 2-aminopurine to approximately the same extent as the in vitro inhibition.Keywords
This publication has 20 references indexed in Scilit:
- Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinaseScience, 1992
- 2‐Aminopurine selectively blocks the transcriptional activation of cellular genes by virus, double‐stranded RNA and interferons in human cellsEuropean Journal of Biochemistry, 1989
- The Mapping of Interferon-Induced Proteins and Phosphoproteins from HeLa S3 CellsJournal of Interferon Research, 1989
- Interferon Induction by Viruses. XVI. 2-Aminopurine Blocks Selectively and Reversibly an Early Stage in Interferon InductionJournal of General Virology, 1988
- 2-Aminopurine Selectively Inhibits the Induction of β-Interferon, c- fos , and c- myc Gene ExpressionScience, 1988
- Protein synthesis initiation factors from human HeLa cells and rabbit reticulocytes are similar: comparison of protein structure, activities, and immunochemical propertiesBiochemistry, 1982
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Phosphorylation of initiation factor eIF-2 and the control of reticulocyte protein synthesisCell, 1977
- An improved heat-stable glutamine-free chemically defined medium for growth of mammalian cellsJournal of Cellular Physiology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970