Detection of a tryptophan radical in the reaction of ascorbate peroxidase with hydrogen peroxide
Open Access
- 15 May 2001
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 268 (10) , 3091-3098
- https://doi.org/10.1046/j.1432-1327.2001.02208.x
Abstract
The reactivity of recombinant pea cytosolic ascorbate peroxidase (rAPX) towards H2O2, the nature of the intermediates and the products of the reaction have been examined using UV/visible and EPR spec...Keywords
This publication has 38 references indexed in Scilit:
- Kinetic study of the inactivation of ascorbate peroxidase by hydrogen peroxideBiochemical Journal, 2000
- Energetics of Cation Radical Formation at the Proximal Active Site Tryptophan of Cytochrome c Peroxidase and Ascorbate PeroxidaseThe Journal of Physical Chemistry B, 1998
- Electrostatic modulation of electron transfer in the active site of heme peroxidasesJBIC Journal of Biological Inorganic Chemistry, 1997
- Kinetic and spectral properties of pea cytosolic ascorbate peroxidaseFEBS Letters, 1996
- Inactivation Mechanism of Ascorbate Peroxidase at Low Concentrations of Ascorbate; Hydrogen Peroxide Decomposes Compound I of Ascorbate PeroxidasePlant and Cell Physiology, 1996
- The homologous tryptophan critical for cytochrome c peroxidase function is not essential for ascorbate peroxidase activityJBIC Journal of Biological Inorganic Chemistry, 1996
- EPR and ENDOR detection of compound I from Micrococcus lysodeikticus catalaseBiochemistry, 1993
- PeroxidasesCurrent Opinion in Biotechnology, 1993
- Molecular cloning and nucleotide sequence analysis of a cDNA encoding pea cytosolic ascorbate peroxidaseFEBS Letters, 1991
- Detection of an oxyferryl porphyrin .pi.-cation-radical intermediate in the reaction between hydrogen peroxide and a mutant yeast cytochrome c peroxidase. Evidence for tryptophan-191 involvement in the radical site of compound IBiochemistry, 1989