Synthesis and Reactivity of 7-Dimethylaminocoumarin-3-isocyanate as a Fluorescent Derivatization Reagent for Alcohols
Open Access
- 1 January 1990
- journal article
- research article
- Published by Pharmaceutical Society of Japan in YAKUGAKU ZASSHI
- Vol. 110 (2) , 155-158
- https://doi.org/10.1248/yakushi1947.110.2_155
Abstract
This paper describes the preparation of 7-dimethylaminocoumarin-3-isocyanate(MACI) having 7-dimethylaminocourmarin as a high sensitive fluorophore together with an isocyanate group as a reacting moiety and a preliminary investigation as a precolumn fluorescence derivatization reagent for alcohols in high performance liquid chromatography (HPLC). Alcohols were derivatized quantitatively into the corresponding fluorescent urethanes by treating with MACI at 80.degree.C for 10 min. The derivatives were subjected to HPLC on an Inertsil ODS (250 .times. 4.6 mm i.d., 5 .mu.M) column with acetonitrile-ethanol-water (50:49:1) as mobile phase and monitored with an excitation wavelength of 382 nm and an emission wavelength of 479 nm. The detection limit of cholestanol was 10 fmol for an injection volume of 10 .mu.l.Keywords
This publication has 1 reference indexed in Scilit:
- Anthracene Isocyanate as a New Fluorescent Label for Compounds with an Alcoholic GroupJournal of Liquid Chromatography, 1982