Multiresidue Method for Isolation and Liquid Chromatographic Determination of Seven Benzimidazole Anthelmintics in Milk

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Abstract
A multiresidue method for the isolation and liquid chromatographic determination of 7 benzimidazole anthelmintics (thiabendazole, oxfendazole, para-hydroxyfenbendazole, fenbendazole sulfone, mebendazole, albendazole, and fenbendazole) in milk is presented. Blank or benzimidazole-spiked milk samples (0.5 mL) were blended with octadecylsilyl (C-18, 18% load, end-capped) derivatized silica packing material. A column made from the C-18/milk matrix was first washed with hexane (8 mL), and then the benzimidazoles were eluted with methylene chloride-ethyl acetate (1 + 2, v/v; 8 mL). The eluate contained benzimidazole analytes which were free from interfering compounds as determined by UV detection (photodiode array, 290 nm). Correlation coefficients of standard curves for individual benzimidazoles isolated from spiked samples were linear (0.989 ± 0.003 to 0.998 ± 0.001) with recoveries ranging from 70 ± 9% to 107 ± 2% for the concentration range (62.5-2000 ng/mL) examined. The inter- assay variabilities ranged from 4 ± 1% to 9 ± 7% with intra-assay variabilities of 3-6%.