Pitfalls of in situ polymerase chain reaction (PCR) using direct incorporation of labelled nucleotides.

  • 1 July 1993
    • journal article
    • Vol. 13  (4) , 1153
Abstract
False positivity is reported of in situ PCR reactions in a direct incorporation assay with digoxigenin-labelled dUTP. It is recommended that in situ hybridization with specific labelled probe replaces the direct incorporation method for the detection of in situ PCR amplicon.

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