Regulation of [3H]forskolm binding to human platelet membranes by GppNHp, NaF, and prostaglandin E1
- 22 April 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 183 (2) , 349-352
- https://doi.org/10.1016/0014-5793(85)80808-5
Abstract
Displaceable binding of [3H]forskolin to human platelet membranes can be detected in the presence of magnesium. There is an increase in the number of [3H]forskolin binding sites when membranes are incubated with GppNHp or NaF in the presence of magnesium. Prostaglandin E1, which stimulates human platelet adenylate cyclase, does not affect the binding of [3H]forskolin in the absence of GppNHp. However, the dose-response curve for the GppNHp-dependent increase in [3H]forskolin binding sites is shifted to lower concentrations in the presence of prostaglandin E1. Prostaglandin E1 potentiates the effect of GppNHp on [3H]forskolin binding most likely by facilitating the binding of the guanine nucleotide at the stimulatory quanine nucleotide regulatory protein of adenylate cyclaseKeywords
This publication has 3 references indexed in Scilit:
- Binding of [3H]forskolin to rat brain membranes.Proceedings of the National Academy of Sciences, 1984
- Activation of Cyclic AMP‐Generating Systems in Brain Membranes and Slices by the Diterpene Forskolin: Augmentation of Receptor‐Mediated ResponsesJournal of Neurochemistry, 1982
- Forskolin: unique diterpene activator of adenylate cyclase in membranes and in intact cells.Proceedings of the National Academy of Sciences, 1981