Disruption of phospholipid asymmetry in erythrocyte vesicles deficient in spectrin
- 1 July 1985
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 9 (7) , 597-606
- https://doi.org/10.1016/0309-1651(85)90051-7
Abstract
The transbilayer distribution of phospholipids in right-side-out and inside-out vesicles derived from human erythrocytes was studied by phospholipase A2 digestion assays and by staining with the fluorescent dye merocyanine 540. In both types of vesicles, the normal asymmetric distribution of phospholipids characteristic of intact cells was disrupted. Because both types of vesicles are deficient in spectrin, the major protein of the cytoskeletal network which normally underlies the membrane, these results support the contention that spectrin is involved in the maintenance of phospholipid asymmetry.This publication has 1 reference indexed in Scilit:
- Syndeins: the spectrin-binding protein(s) of the human erythrocyte membrane.Proceedings of the National Academy of Sciences, 1979