The Cytotoxic Response to Murine Cytomegalovirus. II. In vitro Requirements for Generation of Cytotoxic T Cells
Open Access
- 1 April 1985
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 66 (4) , 757-765
- https://doi.org/10.1099/0022-1317-66-4-757
Abstract
A cytotoxic response to murine cytomegalovirus (MCMV) was obtained by the culture of lymph node cells from mice inoculated with MCMV into both hind footpads 7 days previously. The cytotoxicity was mediated by Thy1.2+, Lyt2+, H-2-restricted effector cells and was virus-specific. Investigation of the in vitro conditions established that T cell proliferation was necessary for optimal generation of cytotoxicity, that proliferation was dependent upon Thy1.2+, Lyt2+ cell populations and that supernatants from concanavalin A-activated spleen cells enhanced the levels of cytotoxicity obtained.This publication has 0 references indexed in Scilit: