Missense mutations in the lacZ gene that result in degradation of beta-galactosidase structural protein

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Abstract
Missense mutations (32) were found among > 200 independently induced mutations in the lacZ gene of Escherichia coli. Twenty of these missense mutations were induced by nitrosoguanidine, and 12 were induced by aminopurine. The lacZ structural protein was endogenously degradable in 7 of the mutant strains; the mutations in these strains were located at only 3 sites in the lacZ gene. Five of the 7 independent mutations were at a single site, and some heterogeneity in the degradation of lacZ protein was observed within these mutant strains.