Subcellular distribution of hydrolase in germinating pea cotyledons
- 1 February 1972
- journal article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 13 (1) , 101-110
- https://doi.org/10.1093/oxfordjournals.pcp.a074707
Abstract
The subcellular distribution of hydrolases in germinating pea cotyledons was investigated by differential and sucrose density gradient centrifugations. Acid phosphatase was present in at least two kinds of subcellular components. One was slightly heavier than mitochondria, and the other was so light that it remained on the upper part of the gradient after sucrose density gradient centrifugation. Protease was localized in the smooth-surfaced microsomal fraction which contained antimycin A-insensitive NADH2-cytochrome c reductase and no RNA. Aryl esterase existed in a soluble form in the cytoplasm.Keywords
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