Identification and mapping of polypeptides encoded by the P3HR-1 strain of Epstein-Barr virus.
- 1 July 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (13) , 4183-4187
- https://doi.org/10.1073/pnas.81.13.4183
Abstract
The Epstein-Barr virus (EBV)-specified polypeptides induced upon viral replication in the human lymphoblastoid P3HR-1 cell line were examined by immunoprecipitation with a high-titer human anti-EBV serum. Twenty-five predominant polypeptides were identified in cell extracts; 18 polypeptides were precipitated from cell-free translation reactions directed by total mRNA. Hybrid selection of mRNA to the BamHI DNA clones of the EBV genome and immunoprecipitation of the corresponding cell-free translation products revealed 98 EBV-specified polypeptides and their coding location along the viral genome. The viral polypeptides that bind reversibly to DNA-cellulose were characterized and the deduced map locations of this functional group of EBV-specified polypeptides is presented.Keywords
This publication has 38 references indexed in Scilit:
- THE PATHWAY OF EUKARYOTIC mRNA FORMATIONAnnual Review of Biochemistry, 1983
- The Biology and Chemistry of Epstein-Barr VirusThe Journal of Infectious Diseases, 1982
- Early DNA-binding polypeptides of Epstein-Barr virusVirology, 1981
- Genome of a mononucleosis epstein-barr virus contains DNA fragments previously regarded to be unique to Burkitt's lymphoma isolatesCell, 1981
- Epstein-Barr virus-induced polypeptides: A comparative study with superinfected Raji, IUdR-treated, and n-butyrate-treated P3HR-1 cellsVirology, 1981
- Transcription and RNA processing by the DNA tumour virusesNature, 1980
- The isolation and characterization of Drosophila yolk protein genesCell, 1980
- Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrateVirology, 1979
- A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybridsPublished by Elsevier ,1977
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973