Separation of human X- and Y-bearing sperm using free-flow electrophoresis.

Abstract
The separation of human X- and Y-bearing sperm was achieved using free-flow electrophoresis. The mobility of X-bearing sperm was faster than that of Y-bearing sperm and the electrophoretic pattern showed the separated two main peaks. One nearer the anode contained only X-bearing sperm, whereas the other was mainly composed of Y-bearing sperm. This result indicates that the zeta potential on the cell surface of X-bearing sperm was higher than that of Y-bearing sperm.

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