Some Properties of Herpes Simplex Virus

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Abstract
Summary: A procedure has been described for the preparation of suspensions of herpes virus which are relatively pure and sufficiently stable to be quite suitable for certain types of experimentation. A 100-fold concentration of the original virus has been achieved along with a 30-fold purification. A high percentage of recovery was obtained and some preparations contained 109 ID50 per mg of nitrogen. In ionic media the maintenance of activity was favored by alkaline conditions. However, the virus was far more stable in water or 0.25 M sucrose than in ionic media. Suspensions in water were examined with the analytical centrifuge and the electron microscope. A sedimentation constant of 1175 S was obtained from which the particle size was estimated to be 96 mμ. In electron micrographs prepared from these suspensions a predominating particle was found which was round in shape and about 116 mμ in diameter. In unshadowed preparations the particles appeared inhomogeneous with regard to electron adsorption indicating the virus is composed of a central structure surrounded by an area of lower density.

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