PRE-PRECIPITATED AND SOLID-PHASE 2ND ANTIBODY COMPARED IN RADIOIMMUNOASSAYS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 26  (3) , 503-507
Abstract
Simple methods for preparing and determining the titer of pre-precipitated [human] 2nd antibody are described. The performance of this type of this type of insolubilized 2nd antibody was compared with that of a commercially available solid-phase 2nd antibody (DASP) for separation of reverse triiodothyronine (rT3) and for thyrotropin radioimmunoassays. Equilibrium time for both reagents was about 30 min, considerably briefer than for the usual 2nd-antibody procedure. Within-assay precision was improved and percentages of nonspecific binding (mean and SD) were lower if pre-precipitated 2nd antibody was used in both assays [rT3 pre-precipitate = 1.48 (SD 0.16), rT3 DASP = 3.76 (SD 0.22); thyrotropin pre-precipitate = 1.45 (SD 0.18), thyrotropin DASP = 3.45 (SD 0.26)]. The cost of DASP was 3-10 times that of pre-precipitated 2nd antibody prepared with commercially available products.

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