Rapid Methods for the Study of both Stable and Unstable Plasmids in Pseudomonas
- 1 June 1981
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 124 (2) , 433-437
- https://doi.org/10.1099/00221287-124-2-433
Abstract
Rapid methods for the analysis of degradative plasmids in Pseudomonas are described. Bacterial lysates prepared with alkaline sodium dodecyl sulfate were vigorously stirred in the presence of antifoam agent. Samples were subjected to agarose gel electrophoresis to detect plasmid DNA. After a short period of centrifugation on a sucrose gradient, the lysates yielded plasmid DNA of sufficient purity for restriction endonuclease digestion without further treatment. The methods have proved most useful in the extraction of previously undetected plasmid DNA, some of which appears to be unstable. [Aromatic compound degradative plasmids pWWO, pWWO-8 and pWW20 of P. putida were among those analyzed by the described procedures.].This publication has 1 reference indexed in Scilit:
- Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5.Journal of Bacteriology, 1978